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Fig. 4. SB removal reveals OA patterning defects. (A) Schematic depicting the experiment in B. (B) In B1, data are expressed as the percentage of embryos with abnormal patterning ±s.e.m. following SB-wash at various time points. Corresponding stages are indicated above the graph. (B2,B3) Phenotype of SB-wash embryos, illuminated with brightfield (B2) and plane-polarized light (B3). (C) QPCR analysis for Nodal (C1) and Gsc (C2) relative expression levels in control, SB-treated and SB-wash embryos. Data are expressed as net {Delta}CT±s.e.m., with controls at hatched blastula (HB) arbitrarily set to zero. Dashed lines indicate the threshold for significance. EG, early gastrula; LG, late gastrula.