Fig. 2. pMAPK antigen, MAPK/GAL4 (MG)-driven GFP and DNA expression in wild-type pupal wings. Anterior is upwards, distal is rightwards. Hours APF is indicated in the upper right-hand corner. pMAPK is in red, MG-driven GFP is in green, DNA is in blue in all panels. (A) pMAPK antigen is predominant in pupal veins (asterisk). (B) At 28 hours APF, pMAPK expression falls in the veins (compare asterisks in A and B). (C) High magnification of distal wing margin at arrowhead in B. MG-driven GFP is present in two lines of cells flanking pro-veins (arrows). (D) pMAPK returns to veins (compare asterisks in B and D). (E) High magnification of boxed area at arrow in A. (F) High magnification of boxed area at arrow in B. pMAPK is predominantly cytoplasmic in both E and F; arrowheads indicate pMAPK staining next to pMAPK-negative nuclei at arrows.