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Fig. 2. Regulation of Gata3 by Pax transcription factors in the pro/mesonephros. Wild-type (A,C) and Pax2-/-Pax8+/- (B,D) embryos were analyzed at the 15-somite stage by in situ hybridization of Gata3 (A,B) and Pax8 (C,D) cRNA probes on adjacent sections. Gata3 expression is specifically lost in the Pax2-/-Pax8+/- nephric duct. nc, nephric cord; nd, nephric duct.