(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. X-TSK functions as a BMP antagonist in Xenopus. (A) Western blot analysis of X-TSK-Myc-His, C-TSK-Myc-His and XBMP4-Flag protein. Each protein was detected by anti-tag antibody. Co-immunoprecipitation of X-TSK-Myc-His or C-TSK-Myc-His and XBMP4-Flag. After immunoprecipitation, bound BMP4 was detected by immunoblotting with anti-Flag antibody. IP, immunoprecipitation. (B) RT-PCR analysis of X-TSK, Noggin or a mixture of both. The numbers above the columns show the amount of X-TSK mRNA in each cap (ng). Plus or minus indicates co-injection or not of Noggin (0.5 ng).