Fig. 7. A molecular interaction between X-TSK and X-delta-1. (A) Immunoprecipitation of X-delta-1 extracellular domain with X-TSK. (B) RT-PCR analysis of acute XESR-1 activation by Notch canonical pathway with or without X-TSK. Animal caps are excised at stage 8.5 and analyzed at stage 9.5. As a positive control, the normal neurula was used (lane 1). Notch ICD (NICD, 1 ng), 1 ng X-delta-1 mRNA, 2 ng X-TSK mRNA, 10 ng X-TSK MO and mixtures thereof were injected into all blastomere at the four-cell stage.