Fig. 2. TUNEL and BrdU analysis of N55 mosaics. (A-C) Increased TUNEL-positive cell death in N55 mutant clones (genotype: ey-FLP; FRT42D N55/FRT42D P[ubi-GFP]) of eye-antennal imaginal discs from third instar larvae. N55 clones are marked by the absence of GFP. (D-F) BrdU labeling of N55 mosaic wing imaginal discs. Note the increased proliferation in tissue adjacent to N55 clones (hs-FLP; FRT42D N55/FRT42D P[ubi-GFP]). (G-I) BrdU labeling of wild-type (wt) clones (hs-FLP; FRT42D +/FRT42D P[ubi-GFP]) in wing imaginal discs serves as a control to D-F. BrdU incorporation is homogeneous inside and outside the clones. (J) Schematic outline of the Vps25 protein and the molecular lesions in Vps25^K2 and Vps25^N55. WHA and WHB are winged-helix domains A and B.