Fig. 3. Accumulation of ubiquitin, N and Dl in vps25 clones, and increased pSTAT immunoreactivity adjacent to vps25 clones. (A-C) Accumulation of ubiquitin in vps25 clones (genotype: hs-FLP UAS-GFP; FRT42D vps25^N55/FRT42D tub-Gal80; tub-Gal4). Note vps25 clones are positively marked with GFP using the MARCM technique (Lee and Luo, 2001). (D-F) Accumulation of N in GFP-marked vps25 clones (genotype as in A-C). (G-I) Accumulation of Dl in GFP-marked vps25 clones (genotype as in A-C). (J-L) Increased N activity in GFP-marked vps25 clones (hs-FLP UAS-GFP; E[spl]m8 2.61-lacZ FRT42D vps25^N55/FRT42D tub-Gal80; tub-Gal4). (M-O) Increased STAT activity inside and outside of vps25 clones (ey-FLP; FRT42D vps25^N55/FRT42D P[ubi-GFP]) by anti-pSTAT labeling. Clones are marked by the absence of GFP.