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Figure 4


Fig. 4. N is required for non-autonomous proliferation of vps25 mosaics, but not for apoptosis. All vps25 clones expressing NDN (vps25/NDN) were labeled with GFP using MARCM. (A-I) vps25/NDN clones have reduced levels of pSTAT (A-C), non-autonomous cell proliferation (BrdU labeling in D-F), and Dl (G-I). The strong BrdU signal in E,F is due to the second mitotic wave. (J-L) Caspase-3*-positive apoptosis is unchanged in vps25/NDN clones. Genotype in A-L: hs-FLP UAS-GFP; FRT42D vps25N55 UAS-NDN/FRT42D tub-Gal80; tub-Gal4. (M) The unmodified GMR-hid GMR-Gal4 eye-ablation phenotype. (N) Overexpression of upd is not sufficient to suppress GMR-hid GMR-Gal4. Genotype: GMR-hid GMR-Gal4/UAS-upd.