Fig. 5. Non-autonomous increase of Diap1 protein levels, and evidence of two cell death pathways. (A-C) Increased levels of Hid protein in vps25 clones (genotype: ey-FLP; FRT42D vps25^N55/FRT42D P[ubi-GFP]) of third instar eye discs. Clones are marked by the absence of GFP. (D-F) Diap1 protein levels are reduced in, and increased adjacent to, vps25 clones (genotype as in A-C). (G-I) BrdU labeling of GFP-marked vps25/Diap1 mosaics (hs-FLP UAS-GFP/UAS-Diap1; FRT42D vps25^N55/FRT42D tub-Gal80; tub-Gal4). Overexpression of Diap1 does not block non-autonomous proliferation. (J-L) Caspase-3^*-labeling of GFP-marked vps25^N55/Diap1 mosaics. Caspase-3^* is completely blocked. Genotype as in G-I. Scale bar: 100 µm. (M-O) Caspase-3^*-labeling of GFP-marked vps25 ark double mutant mosaics. Caspase-3^*-dependent cell death is detectable in vps25 ark clones (hs-FLP UAS-GFP; FRT42D vps25^N55 ark^H16/FRT42D tub-Gal80; tub-Gal4). Scale bar: 100 µm. (P) Adult eyes of ey-FLP-induced mosaics of vps25 ark double mutants are severely overgrown and folded. Note that vps25 ark clones (ey-FLP; FRT42D vps25^N55 ark^H16/FRT42D P[w⁺]) are absent (marked by lack of red pigment).