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Figure 6


Fig. 6. JNK contributes to the apoptotic phenotype of vps25 clones. (A-C) Increased JNK activity in vps25 clones (genotype: ey-FLP; FRT42D vps25N55/FRT42D P[ubi-GFP]) shown by anti-pJNK labeling. Clones are marked by absence of GFP. (D-F) pJNK labeling of GFP-marked vps25/Diap1 mosaics (hs-FLP UAS-GFP/UAS-Diap1; FRT42D vps25N55/FRT42D tub-Gal80; tub-Gal4). (G-I) Caspase-3*-labeling of GFP-marked vps25/Puc mosaics (hs-FLP UAS-GFP; FRT42D vps25N55/FRT42D tub-Gal80; tub-Gal4/UAS-puc). Scale bar: 100 µm. (J-L) Caspase-3*-labeling of GFP-marked vps25 ark/Puc mosaics (hs-FLP UAS-GFP; FRT42D vps25N55 arkG8/FRT42D tub-Gal80; tub-Gal4/UAS-puc). Note, the clones marked with GFP are severely enlarged. Scale bar: 100 µm.