Fig. 7. Increased Hippo signaling, but not cell competition, controls apoptosis in vps25 clones. (A-C) Caspase-3^*-labeling of vps25 mosaics (genotype: ey-FLP; FRT42D vps25^N55/FRT42D arm-lacZ M(2)) in a Minute (M) background. Caspase-3^* activity is unaffected. Clones are marked by the absence of ß-Gal staining. (D-F) Caspase-3^*-labeling of GFP-marked vps25/dMyc mosaics (hs-FLP UAS-GFP; FRT42 vps25^N55/FRT42D tub-Gal80; tub-Gal4/UAS-dMyc). Caspase-3^*-activity is unaffected. (G-I) Expanded (Ex) labeling of GFP-marked vps25 mosaics (hs-FLP UAS-GFP; FRT42D vps25^N55/FRT42D tub-Gal80; tub-Gal4). Ex protein levels are reduced in vps25 clones (arrow), indicating increased Hippo activity. (J-L) Caspase-3^*-labeling of GFP-marked vps25 hippo double mutant mosaics (hs-FLP UAS-GFP; FRT42D vps25^N55 hippo^3D/FRT42D tub-Gal80; tub-Gal4). Caspase-3^* activity is blocked.