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Figure 3


Fig. 3. smp and PCNA transcript distributions showing that smp is expressed in CNS proliferating cells. (A) Drawing indicating section plans. (B-G) 34/35-somite embryos were sectioned after WMISH with a smp (B,D,F) or a PCNA (C,E,G) probe. smp and PCNA expression domains in the CNS were compared in the telencephalon (Tel), the diencephalon (Di) and the mesencephalon. Telencephalic (B,C) and diencephalic (D,E) sections show that the dorsal and peri-ventricular domains are positive for smp and PCNA. On diencephalic sections, eyes show ciliary marginal zones positive for both smp and PCNA. (F,G) Sections through the mesencephalon with smp and PCNA staining at the margin of the optic tectum (ot). Scale bars: 60 µm.