Fig. 5. Raldh1 and Raldh3 are not required to establish or maintain dorsoventral patterning of the retina. All panels consist of frontal sections of eyes at either E10.5 (A-D) or E14.5 (E-H). (A) Tbx5 mRNA in a wild-type embryo. (B) Vax2 mRNA in a wild-type embryo. (C) Double in situ hybridization showing both Tbx5 and Vax2 mRNA in a Raldh1^-/-;Raldh3^-/- embryo. (D) Vax2 mRNA in limited-rescue Raldh1^-/-;Raldh2^-/-;Raldh3^-/- triple mutant embryo. (E-H) Detection of ephrin B2 mRNA (E,F) and Ephb2 mRNA (G,H) in wild-type and Raldh1^-/-;Raldh3^-/- embryos reveals that the double mutant still expresses both genes at relatively normal levels in their correct dorsoventral positions. D, dorsal; V, ventral.