Fig. 4. Localization of DGN-1 in epithelia and neurons, but not muscle. (A) In early embryos (pre-lima stage) before BM formation, DGN-1 (red) is distributed around the surface of many epithelial and neural precursors. (B) As BMs assemble (lima to comma stages), identified by staining for NID-1/nidogen (green), DGN-1 (red) redistributes to the basal surface (arrowheads). (C) In late embryos (pretzel stage), DGN-1 (red) is prominent in marginal cells (mc) and the gonad primordium (gp), and localizes to basal surfaces marked by NID-1 (green). Hypodermal DGN-1 colocalizes with NID-1 at the edges of the body wall muscles (hme). (D) DGN-1 (red) and EPI-1/laminin-B (green) colocalize in the gonad primordium of late embryos (pretzel stage). (E) DGN-1 (green) is prominent in spermatheca (st), gonad sheath (sh) and distal tip cells (dtc) of the gonad and the vulval epithelium (vul). (F,G) In the gonad and vulva, DGN-1 (red) localizes to the BM surface (NID-1, green). (H,I) DGN-1 (green) is at the basal surface of the rectal epithelium (H, rect) and the excretory cell (I, exc). (J) DGN-1 (green) in the PVP neurons. (K) DGN-1 (green) localization in the hypodermis (hyp) and excretory cell, but not in body wall or pharyngeal muscle, visualized by staining of MHC-A myosin in muscle M-lines (red). (L) DGN-1 (green) is seen weakly throughout the hypodermis covering muscle and is slightly concentrated adjacent to the body wall muscle (arrowheads), but not in the underlying muscle (red). Scale bar: 10 µm.