Fig. 5. The delay in the migration of LRN/ECN neurons expressing DN cadherin constructs is observed before crossing of the ventral midline by control neurons expressing only EGFP. Flat whole-mount preparations electroporated with expression vectors encoding EGFP (A,B), EGFP+EGFP-NCAD(t) (C,D) at 1.5 div. (B,D) Enlarged views of migrating neurons on the ipsilateral side near the FP in A,C, respectively. Whereas the control neurons with only EGFP reach the FP at this time (A,B), the neurons expressing EGFP+EGFP-NCAD(t) do not (C,D). Broken lines labeled VM indicate the position of the ventral midline. Scale bars: 400 µm in A,C; 50 µm in B,D.