Fig. 3. Testicular degeneration in Bmp4^S2G/S2G adults. (A) Isolated testes from age matched wild-type and Bmp4^S2G/S2G adult males. (B,C) Hematoxylin and Eosin-stained sections from age matched adult testis. (D-G) DAPI staining (D,F) and TUNEL staining (E,G) of sections from age matched adult testes. (H,I) Hematoxylin and Eosin-stained P6 testis sections from wild-type and mutant littermates. (J) Western blots of equivalent amounts of protein from wild-type and mutant P6 testes probed with an antibody directed against the mature domain of BMP4. The blot was reprobed for actin as a loading control.