Fig. 3. Conservation of TAGteam sites in pre-cellular blastoderm genes is variable. (A) Alignment of bnk promoter sequences for Drosophila melanogaster (mel), yakuba (yak), ananassae (ana), pseudoobscura (pse), willistoni (will), mojavensis (moj), hydei (hyd), virilis (vir) and Zaprionus tuberculatus (Zap). Grey shading indicates at least 50% identity among nucleotides at sites present in at least two species. The TATA site is boxed in blue, while TAGteam sites are boxed in red. An arrow denotes the transcription start site. (B) Phylogenetic relationships, estimated divergence times (Kwiatowski et al., 1994; Powell and DeSalle, 1995; Russo et al., 1995), and number of TAGteam sites in bnk for the nine species. Parentheses indicate values from species for which less DNA sequence was available. (C) Schematic of a five-species alignment for the promoter-proximal upstream 400 bp of five pre-CB genes, and for TAGteam-rich enhancer elements further upstream in two cases (distances not to scale). Circles represent CAGGTAG sites, squares tAGGTAG and diamonds CAGGcAG. Overlapping symbols indicate overlapping motifs. In the rare event that a TAGteam site appeared to be replaced by its reverse complement, that complement was diagrammed as a separate site.