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Figure 4


Fig. 4. Pol I transcription is reduced in basonuclin-RNAi transgenic oocytes. Run-on assays were performed on growing oocytes (14 days) from control (A,C,F) and line T50 (B,D,G) in the absence (A,B) and presence (C,D) of 100 µg/ml {alpha}-amanitin. The incorporated BrdU was visualized by AlexFluor-488-labeled anti-BrdU antibody; nucleoplasm staining is indicated by arrowheads and that of nucleolus by arrows. The fluorescence intensity of nucleolus in run-on with {alpha}-amanitin was measured from micrographs with ImageJ (non-T, n=14; T50, n=10), and the average in arbitrary units is shown (E). Nucleoli of 20 oocytes from each group were optically sectioned (F, non-T control; G, T50), and the transcription foci (brightly labeled spots) were scored. The average number of foci per oocyte is shown (H). *P<0.05; **P<0.001.