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Figure 2


Fig. 2. Neural crest-specific knockout of Cx43 in Cx43-WCKO embryos. (A) Low-power, sagittal section of a wild-type E9.5 embryo demonstrating first, second and third pharyngeal arches. (B,C) Immunostaining for Cx43 (green stain) in high-power images of the first pharyngeal arch (PA1) (B) and the third pharyngeal arch (PA3) (C), in the E9.5 wild-type embryo. (D) Low-power image of an E9.5 Cx43-WCKO embryo. (E,F) High-power images of PA1 (E) and PA3 (F) in the E9.5 Cx43-WCKO embryo immunostained for Cx43. Cx43 signal is nearly absent in PA1 and PA3 in the Cx43-WCKO embryos in contrast to wild-type littermates. (G,H) Heart sections from E10.5 wild-type (G) and Cx43-WCKO (H) embryos immunostained for Cx43. Expression of Cx43 in the Cx43-WCKO heart is preserved at this stage. Propidium iodide nuclear staining is in red. Scale bar: 320 µm for A,D; 20 µm for B,C,E,F; 40 µm for G,H.