Fig. 5. Reduction of the pool of ENS progenitors in
Edn3ls/ls embryos. (A) Whole-mount preparation
of gut from Edn3+/+; TgWnt1Cre;
R26YFPStop embryos immunostained for YFP. Box indicates
the part of gut used to generate the intestinal acute cultures. (B)
Bars represent the fraction of double positive
(SOX10+YFP+) cells relative to the total population of
neural crest-derived (YFP+) cells in acute intestinal cultures
established from embryos wild-type (red) or heterozygous (blue) and homozygous
for Edn3ls (green).