Fig. 6. Reduced EDN3 signalling decreases the proliferation of specific subpopulations of enteric neural crest cells. (A) Whole-mount preparation of gut from Edn3^+/+; Tg^Wnt1Cre; R26^YFPStop embryos immunostained for YFP. Box 1 indicates the part of gut used to generate the intestinal acute cultures examined in C and D. Box 2 indicates the area of the gut analysed in B and E. (B) Confocal image of an embryonic gut region corresponding to box 2 of A. Gut was immunostained for BrdU (red) and YFP (green). (C) Bars represent the fraction of BrdU⁺YFP⁺ cells relative to the total population of neural crest-derived (YFP⁺) cells in acute intestinal cultures from wild-type (red), Edn3^+/ls (blue) and Edn3^ls/ls (green) embryos. (D) Percentage of BrdU⁺SOX10⁺ cells within the total population of SOX10⁺ cells in acute intestinal cultures from wild-type, Edn3^+/ls and Edn3^ls/ls embryos. (E) Fraction of BrdU⁺YFP⁺ cells relative to the total population of YFP⁺ cells at the migratory wavefront of enteric neural crest cells.