Fig. 7. Increased neuronal differentiation of enteric neural crest cells at the migratory front. (A,B) Whole-mount preparations of guts from E11.5 Tg^Wnt1Cre;R26^YFPStop embryos wild-type (Edn3^+/+; A) or homozygous for the ls allele (Edn3^ls/ls; B); guts were immunostained for YFP (green) and TuJ1 (red). Images correspond to the front of enteric neural crest cell migration (part of box 2 of the gut shown in Fig. 6A). (C) Bars represent the fraction of double-positive (TuJ1⁺YFP⁺) cells within the total population of YFP⁺ cells present at the front of migration of wild-type (red), Edn3^+/ls (blue) and Edn3^ls/ls (green) embryos.