Fig. 1. Cortical interneurons orient in many directions in multiple zones. (A) Semi-schematic representation of a transverse section of an E15.5 GAD67-GFP mouse cortex indicating the depth of tangential sections. Numbered blue lines represent the approximate level of the cortex from which the corresponding tangential sections in B were obtained. Dotted line indicates the central region of the sections indicated by boxes in B. (B) GFP neurons in E15.5 serial tangential sections, lined from pial to ventricular side. (C) Higher magnification confocal images of the boxed areas in B. Two images were taken from each section by changing the depth of focus. C1, C3, C5, C8 and C10 were taken from the dorsal part of tangential slices numbered 1, 2, 3, 5 and 6 in B, respectively. C2, C4, C6, C9 and C11 were from the ventral part of slices numbered 1, 2, 3, 5 and 6 in B, respectively. GFP neurons orient in many directions in the MZ, CP, SP and VZ. M, medial; V, ventral; R, rostral. Scale bars: A, 200 µm; B, 400 µm; C, 40 µm.