Fig. 2. Time-lapse analysis of MDT migration of interneurons in the VZ. (A) Schematic of time-lapse imaging in a flat-mount preparation. Dorsal cortex was excised and flat-mounted with the ventricular side up. (B) Confocal images of interneurons in the VZ of an E13.5 mouse viewed from the ventricular side. The number in the top right corner is the time in minutes. Neurons indicated by arrowheads of the same colour represent the same neurons. (B') Tracks of the four neurons shown in B. Each dot corresponds to the neurons shown by arrowheads in B and illustrates their positions plotted at 5 minutes intervals. Arrows indicate the direction of migration. R, rostral; L, lateral. Scale bar: 20 µm.