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Figure 6


Fig. 6. Dispersion of DiD-labelled GFP neurons in the MZ after DiD injection into the parietal cortex. (A) Schematic of the experimental paradigm used. DiD-labelled GFP neurons in the MZ were analysed after an appropriate survival period by preparing flat-mount cortical preparations. About 70% of DiD-labelled neurons expressed GFP (n=352/485 DiD-labelled cells, 12 brains). The remainder may include Cajal-Retzius cells (Bielle et al., 2005; Takiguchi-Hayashi et al., 2004). (B,C) Distribution of DiD-labelled neurons in flat-mount preparations one and a half days (E17.0; B) or three days (E18.5; C) after injection. (B1) Dorsal view of the area around the injection site. (B2,B3) Higher magnification of the DiD-labelled GFP neurons indicated by straight lines in B1. These neurons extended an unbranched (B2) or branched (B3) leading process and a short trailing process. Comparison of fluorescence in a single optical section demonstrates that this DiD-labelled cell (inset, magenta) expresses GFP (green). (C1) Dorsal view of the area around the injection site. (C2-C4) Higher magnification of the DiD-labelled GFP neurons indicated by straight lines in C1. These neurons showed typical morphology of migrating neurons in the MZ extending a leading process (C2), occasionally extending a thick but short process (C3) that appears to elongate toward the CP (data not shown). Neurons having several long processes, and granular structure in the trailing process were also observed (C4). (D) Distribution of DiD-labelled GFP neurons in flat-mount preparations one and a half (E17.0; black circles) and three days (E18.5; white circles) after injection. The neurons situated >600 µm or >800 µm from the injection site were plotted for E17.0 or E18.5 brains, respectively. Pooled data from two (E17.0) or six (E18.5) independent experiments. The lack of data medial to the injection site is due to folding of the cortex. OB, olfactory bulb; R, rostral; C, caudal; L, lateral. Dotted lines, flat-mount outline. Scale bars: in B1,C1, 200 µm; in B2, B3, 10 µm; in C2-C4, 10 µm.