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Figure 8


Fig. 8. Distribution and characterization of DsRed-labelled cells 3.5 days after electroporation (E15.5). (A) Distribution of DsRed-labelled cells (arrows) in the dorsal cortex in a coronal section. Schematic illustrates GE-directed electroporation at E12.0 (inset). (B,C) Immunohistochemical characterization of DsRed cells (magenta) in the neocortex. Most DsRed cells in the dorsal cortex were positive for both GAD67-GFP (green in B; 98.1±1%; n=207/210 DsRed cells, three brains) and LHX6 (green in C; 91.4±7%; n=154/163 DsRed cells, five brains). Dotted lines indicate the section outline. D, dorsal; L, lateral. Scale bars: A, 200 µm; B,C, 10 µm.