Fig. 4. UNC-63-containing nAChRs are required to implement the DMPP signal.
(A) Survival on 0.75 mM DMPP. Error bars represent s.e.m.
(n
3). Punc-63::unc-63: extrachromosomal array carrying
an unc-63 genomic fragment; Pmyo-3::unc-63: muscle specific
promoter driving the expression of an unc-63 cDNA.
unc-63(kr13);Punc-63::unc-63, unc-38(x38) and unc-29(e1072)
are significantly less DMPP resistant than unc-63(kr13) (*:
P< 0.05, Mann-Whitney test). Pmyo-3::unc-63 does not
rescue unc-63(kr13) DMPP resistance while it restores wild-type
locomotion and levamisole sensitivity (see Fig. S1 in the supplementary
material). (B-D) unc-63 expression profile. Confocal picture
of a transgenic larva expressing Punc-63::unc-63-SL2-GFP. unc-63 is
expressed in body-wall muscles (B, arrow), head muscles (C, arrowhead), and in
many neurons in the head (C) and the ventral cord (D, arrow). Scale bar: 20
µm. (E) unc-63(kr13) does not alter the timing of L1/L2 and
L2/L3 molts. Each dot represents the percentage of worms pumping at a given
time (n>25 individuals). Broken lines indicate the timing of
wild-type events.