Fig. 2. Rescue of vCrz-cell death by transgenic expression of p35. (A) Control 1-day old pupa (UAS-lacZ/+; Crz-gal4/+). (B) One-day-old pupa expressing p35 in Crz neurons (UAS-lacZ/+; Crz-gal4, UAS-p35/+). Persistent vCrz neuronal somata and ascending projections are designated by arrows and arrowheads, respectively. (C-F) Progressive removal of lacZ-marked vCrz neurons (UAS-lacZ/+; Crz-gal4/+) during metamorphosis. White prepupae were aged at 25°C for durations designated in each panel, and then processed for X-gal staining. (C) White prepupa (n=5). (D) 2 hours APF (n=6). (E) 3 hours APF (n=4). Arrow points to discontinued projection. (F) 6 hours APF (n=9). Faint dots (arrow) most probably come from residual ß-galactosidase activity rather than from live cells. Scale bar: 100 µm.