Fig. 8. Robo1, calbindin and GAD65 immunohistochemistry of cells from the ganglionic eminence. (A) Image of a wild-type coronal section showing robust Robo1 staining in the mantle zone of the ganglionic eminence (GE) and in two bands in the marginal zone (MZ) and lower intermediate zone (IZ) of the developing cortex. At high magnification, the receptor was clearly localized in some individual cells, especially near the corticostriatal notch, that showed features of migrating neurons (boxed area is shown at higher magnification in A'). (B) Similar to Robo1 staining, calbindin immunoreactivity was localized chiefly in the GE, and in the corridors within the cortex used by migrating cortical interneurons, the MZ and lower IZ. (C-E) Dissociated GE cell cultures prepared from an E15 wild-type animals were co-stained with Robo1 (C) and GAD65 (D), showing that GABAergic cells in this part of the ventral telencephalon express the receptor (E, a composite of C and D). Scale bars: 200 µm in A,B; in E, 10 µm in A' and 35 µm in C-E.