Fig. 8. 12/101 staining of regenerating tails in wild-type and pax7EnR transgenic tadpoles. (A) The flowchart in the experiment of repeated tail amputation. The first heat shock is given 3 hours before the first amputation and heat shocks are given daily thereafter. The distal 50% tail was cut in the first tail amputation. The second tail amputation was performed at 14 dpa at the site of distal 75% of the first regenerated tail. The purple and blue tails indicate the primary and secondary regenerated tail, respectively. (B-F) 12/101 antibody staining of the regenerated tails. (B) Wild-type tails 14 days post first amputation. (C) pax7EnR transgenic tails 14 days post first amputation. (D) Wild-type regenerated tails, 14 days after second amputation. (E,F) pax7EnR regenerated tails 14 days after second amputation. (G,H) Transverse section of second regenerated tail in wild type (G) and pax7EnR transgenic tadpoles (H). Section level is indicated by the black lines in D,E. Abbreviations: sc, spinal cord; not., notochord; mus, muscle. Scale bars: 500 µm in B-F; 50 µm in G,H.