Fig. 2. Comparison of H3-K9 acetylation, H3-K4 methylation and H3-K27 trimethylation in Hoxb8 genomic surrounding at 12.5 dpc between anterior and posterior tissues. (A) Comparative analysis of H3-K9 acetylation at regions 2, 7, 8 and 9. (Left) Whole-cell lysates (WCE) prepared from anterior and posterior parts of 12.5 dpc embryos were subjected to ChIP analyses by using anti-acetylated H3-K9. Amounts of immunoprecipitated genomic DNA (A+ and P+) by anti-acetylated H3-K9 subjected to PCR were equivalent to that of `Input' DNA loaded in lane 1. Mock-immunoprecipitated DNA (A- and P-) derived from the same volume of the chromatin fraction as used for anti-acetylated H3-K9 immunoprecipitation were subjected to the PCR. (Right) Schematic comparison of H3-K9 acetylation between anterior and posterior tissues. The relative quantity of each genomic region in immunoprecipitated genomic DNA from anterior and posterior tissues was estimated by referring to `Input' DNA isolated from the initial lysates and enrichment values against the initial lysate are represented by the black and gray bars, respectively. (B) Comparative analysis of di- and trimethylation of H3-K4 at the region 2, 7, 8 and 9. Representative results (left) and schematic summary (right) are shown. (C) Comparative analysis of trimethylation of H3-K27 at the region 2, 3 and 8. Representative results (left) and schematic summary (right) are shown.