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Figure 3


Fig. 3. Persistent Hes1 expression in the roof plate and the floor plate. (A-X) In situ hybridization for Wnt1 and Shh, Hes1 immunostaining and PI staining were performed with embryos at E9.5, E11.5 and E13.5. Cells in the roof plate and the floor plate persistently express Hes1 protein at high levels during this period. Scale bar: 50 µm.