Fig. 5. Anti-BDNF decreases synapse density per dendrite branch. (A) Reconstructions of a sample tectal neuron imaged at 0, 4 and 24 hours further illustrate the onset of anti-BDNF effects on postsynaptic specialization density and dendritic arbor morphology. Scale bar: 20 µm. (B,C) High-magnification images of individual dendritic branches in (B) control and (C) anti-BDNF treated tadpoles compare changes in PSD95-GFP puncta per branch, over the 48 hour imaging period. Scale bar: 5 µm. (D) A measure of the effects of anti-BDNF on postsynaptic specialization density per branch was obtained by counting the number of PSD95-GFP puncta at each observation interval in branches with lifetimes of more than 48 hours (that were present at all observation time points, from 0 to 48 hours). Data were normalized for the change in branch length and are presented as the percent of its initial value at the time of treatment compared with control. Bars indicate s.e.m. n=25 branches in control and n=25 in anti-BDNF. ^*P0.05, ^**P 0.005 compared with control.