Fig. 6. Temporal axons stop in non-graded striped ephrin patterns. (A) Stop zones of temporal axons in non-graded patterns printed with 8 µg/ml ephrinA5. Antibody-stained ephrin is shown in red; phalloidin-stained axonal actin in black. In the pattern consisting of 0.3 µm thick stripes, growth cones stop later than in the pattern of 1.8 µm stripes. Scale bar: 100 µm. (B) The position of the axonal stop points in three different stripe patterns is plotted against the stripe thickness for patterns printed with 8, 4 and 2 µg/ml. Mean stop points were 49.1±2.3 µm (stripe thickness 0.3 µm, n=5), 37.4±3.0 µm (0.6 µm, n=6), 28.3±1.7 µm (1.8 µm, n=6) for 8-µg/ml patterns, 83.7±7.8 µm (0.3 µm, n=6), 66.0±4.3 µm (0.6 µm, n=7), 48.8±4.2 µm (1.8 µm, n=10) for 4 µg/ml patterns and 122.4 µm (0.3 µm, n=2), 93.7 µm (0.6 µm, n=3), 82.6 µm (1.8 µm, n=3) for 2 µg/ml patterns. Error bars indicate standard error. Due to the data examination method, no standard error could be calculated for the mean stop point position in the patterns printed with 2 µg/ml (marked with a stars, for details see Materials and methods). P<0.001 by ANOVA for 8 µg/ml patterns and P<0.006 for 4 µg/ml patterns. Studens t-test for small sample sizes was applied to compare the mean stop points in patterns with the same stripe thickness printed with 8 and 4 µg/ml: P<0.001 (stripe thickness 0.3 µm), P<0.001 (stripe thickness 0.6 µm), P<0.003 (stripe thickness 1.8 µm).