Fig. 6. Cloning and sequence of Dachs. (A) Localization of dachs on 2L. P elements used for male recombination are indicated above the chromosome. Horizontal arrows indicate the result of recombination, placing dachs distal to each insertion. Deficiencies used to map dachs are shown below. (B) Protein domains, transcript and gene structure of dachs. Exons are indicated as boxes and are numbered. Intervening introns appear as black lines. The locations of mutations in dachs alleles are indicated. (C) Amino acid sequence of Dachs. The conserved myosin head domain is in bold. Underlining identifies motifs discussed in the text: 1, coiled-coil domain; 2, ATP-binding domain; 3, unique insert in myosin head domain; 4, actin-binding domain; 5, active thiol domain; 6, IQ camodulin-binding domain; 7, putative transmembrane domain. The locations of mutations are indicated above: d^GC2, inframe deletion indicated by broken line; d^GC13, 11 bp deletion predicted to truncate at the indicated aa; d²¹⁰, point mutation introducing a stop codon; d¹, insertion of a blood retrotransposon.