Fig. 2. calderón embryonic phenotype. (A-C) Lateral view (anterior left, dorsal up) of wild-type (A), cald^R107/cald^R107 (B), and cald^EP1072/cald^EP1072(C) first instar larvae. Mutant embryos exhibit the characteristic U-shaped phenotype due to a defective germ band retraction. (D) Lateral view of a cald^R107 embryo driving the expression of GFP in the calderón pattern. (E-H) Lateral view of embryos showing the distribution of CG13610 transcripts. Note reduced expression levels in cald^R107 (F) or cald^EP1072 (G) mutant embryos, when compared with heterozygous cald^R107 (E) or cald^R107/cald^EP1072 (H) embryos. Arrows point to the amnioserosa. Double in situ hybrdization and antibody staining against ß-Gal protein (brown in E) was performed to identify homozygous and heterozygous cald embryos.