Fig. 5. The role of calderón activity in Minute mediated cell competition. (A) Diagram depicting the induction of Minute⁺ cald mutant clones (upper cell) and their Minute^- cald⁺ twins (lower cell) after a mitotic recombination event. (B-G,I) Clones are marked by the absence of the ß-Gal marker (grey). Twins are marked by the presence of two copies of the ß-Gal marker (white). (B,D,F) Minute⁺ control clones visualized 24 (B), 48 (D) and 72 (F) h after induction. Note all twin Minute^- homozygous clones have already disappeared 48 h after induction. (C,E,G,I) cald Minute⁺ clones visualized 24 (C), 48 (E) and 72 (G,I) h after induction. Some twins (Minute^- homozygous and wild type for cald function) are recovered even 72 h after induction (magnified in I). (H) Graph plotting the ratio of Minute^-/Minute⁺ control clones (in black) and the ratio of Minute^-/Minute⁺ cald mutant clones (in red). 24 h after clone induction: number of Minute⁺ clones: 522; number of Minute⁺ cald clones: 253. 48 h after clone induction: number of Minute⁺ clones: 507; number of Minute⁺ cald clones: 187. 72 h after clone induction: number of Minute⁺ clones: 58; number of Minute⁺ cald clones: 81.