Fig. 7. calderón expression is regulated by the Insulin pathway. (A-E) Lateral view (anterior left, dorsal up) of embryos showing the distribution of cald transcripts in various genetic backgrounds. (A,C,E) Activation of the InR pathway up-regulates cald expression. Stage 13 embryos showing cald transcripts in wild-type (A), ptc-Gal4:UAS-dS6K (C) and Ubx-Gal4:UAS-dS6K (E) backgrounds. At this stage, wild-type embryos show cald expression only in amnioserosa and a group of cells of the CNS. Activation of InR pathway in ectodermal cells in ptc (C) and Ubx (E) domains up-regulates cald expression in the ectoderm. (B,D) Down-regulation of the InR pathway eliminates cald expression. Stage 11 embryos showing cald transcripts in wt (B) and Kr-Gal4:UAS-PI3K92E-Dp110^DN (D) backgrounds. Note reduced expression of cald in amnioserosa cells in D (red arrow). (F) Down-regulation of InR pathway in amnioserosa cells phenocopies cald mutants. Lateral view of Kr-Gal4:UAS-PI3K92E-Dp110 ^DN first instar larva. These larvae exhibit the characteristic U-shaped phenotype of cald mutants (compare Fig. 2B with C). (G,H) Third instar wing discs showing cald transcripts in wild-type (G) and MS1096-Gal4:UAS-PI3K92E-Dp110^DN (H) backgrounds. MS1096-Gal4 is expressed at higher levels in the dorsal compartment (d) of the wing pouch. The boundary between dorsal (d) and ventral (v) compartment is shown.