Fig. 2. Src64 mutants exhibit ring canal morphology defects. (A) Confocal projections of stage 10 WT, Src64 mutant (Src64^KO, Src64¹⁷), or rescued Src64 mutant ([Src64^WT]/+;Src64^KO or [Src64^WT]/+;Src64 ¹⁷) egg chambers stained with anti-Hts-RC to visualize ring canals. (B) Average size ring canals labeled with anti-Hts-RC. (C) Partial confocal projections of stage 10 egg chambers labeled with phalloidin (green) to visualize filamentous actin and propidium iodide (red) to visualize DNA. Arrows and brackets indicate nurse cell fusion events. Scale bars: 10 µm.