Fig. 4. Src64^LOF egg chambers exhibit packaging defects. (A-E,G) Partial confocal projections of Src64^LOF egg chambers labeled with phalloidin (green) to visualize filamentous actin and propidium iodide (red) to visualize DNA. (A) Src64¹⁷ egg chamber containing 30 nurse cells (NC) + two oocytes (OO) (32 germ cells (gc) total). (B) Src64¹⁷ egg chamber containing 60 NC + 4 OO (64 gc total). (C) Src64^KO egg chamber containing 22NC+1OO (right) next to egg chamber containing 8NC+1OO (32 gc total). (D) Src64^KO egg chamber containing 24NC+2OO (right) next to egg chamber containing 6NC (32 gc total). (E) Src64^KO ovariole. (F) hsFlp/+;Src64¹⁷,FRT2A/GFP,FRT2A egg chamber with too many germ cells labeled with phalloidin (red) and anti-GFP (green). Src64 mutant cells lack GFP. (G) Src64^KO egg chamber containing too many cells as well as catastrophic membrane collapse. (H) w,hsFlp/+;Src64¹⁷,FRT82B/Src64¹⁷,FRT82BGFP egg chamber labeled with anti-GFP (green) and phalloidin (red). Half the cells in the egg chamber are labeled with GFP (green) and half are unlabeled, indicating that the cells come from independently derived germline cysts that were packaged together. Scale bars: 10 µm.