Fig. 5. Increasing Src64 activity leads to packaging defects. Partial confocal projections of egg chambers containing too few (^*) or too many (^**) cells. (A) [Src64^Y547F]-expressing egg chambers labeled with phalloidin (green) to visualize filamentous actin and propidium iodide (red) to visualize DNA. (B) Csk^D589N egg chambers labeled with phalloidin. (C) Csk^E481K mutant tissue is marked by the absence of GFP (green). Nuclei are labeled with propidium iodide (red). The youngest egg chamber includes labeled and unlabeled cells; thus Csk^E481K germline mutation leads to packaging defects. Scale bars: 10 µm.