Fig. 8. Src64 is activated on ring canals. (A) Wild-type ovariole stained with phalloidin to visualize filamentous actin (green) and anti-pY434 activation-specific Src64 (red). (B) Rescue of Src64 activation (right) by expressing wild-type ([Src64^WT]/+;Src64¹⁷), but not kinase dead ([Src64^KD]/+;Src64¹⁷) Src64 in Src64¹⁷ mutants. Filamentous actin is labeled with phalloidin (left). (C) Reducing Csk in Src64 hypomorphs can suppress the loss of Src64 activation at the ring canal. Src64¹⁷,Csk^1T6/Src64¹⁷ egg chamber stained with phalloidin and anti-pY434. Boxes (green or pink) surround a ring canal that is weakly stained with anti-pY434. Below is a larger magnification view of the same ring canal.