Fig. 4. B8.12 descendants coalesce into the gonad. (A) A gastrula embryo with DiI-labeled B7.6 cells (arrowhead). (B) A tailbud embryo with DiI signals in the B8.11 (arrowhead) and B8.12 (arrow) cells. (C) A stage 6 juvenile, in which the B7.6 cells were DiI labeled, were stained for CiVH (green). The arrowhead indicates the primitive gonads. Five DiI-labeled cells (arrowheads) that were also CiVH positive coalesced into the gonads (inset). (D) A larva in which B7.6 blastomeres were DiI-labeled and the distal part of the tail containing the B8.12 descendants was cut off. The DiI signals remained in the B8.11 cells of the tail (arrowhead). (E) A stage 5 tail-cut juvenile. The DiI-positive B8.11 cells were present on the gut wall (arrowhead). Owing to the capture of a faint DiI signal, the auto-fluorescence from the tail debris was relatively prominent (arrow). No CiVH-positive cells were detected in this juvenile (data not shown).