Fig. 5. CiVH RNA and protein are inherited by the B8.12 cells through their release from the CAB. Embryos were stained for CiVH protein (magenta) and CiVH RNA (green), and their nuclei were labeled (blue in J,O). (A-E) A 32-cell stage embryo; (F-J) a gastrula embryo; (K-O) a mid-tailbud embryo. (B-E,G-J,L-O) Enlarged views of A,F,K. (A,B,F,G,K,L) Nomarski optics. (C-E) CiVH protein and RNA concentrated to the CAB during cleavage stages (arrowheads). (H-J) Some CiVH protein (white arrows) and RNA (yellow arrows) diffused together from the CAB (arrowheads) in gastrula stage embryos. (M-O) In tailbud embryos, although CiVH protein and RNA colocalized in the B8.11 cells (arrowheads), they were separated in the cytoplasm within the B8.12 cells (white and yellow arrows, respectively).