Fig. 3. No changes in PC proliferation and cell cycle exit in the Ebf2 null cerebellar primordium. Immunohistochemistry (A-F) and in situ hybridization (G,H) on the E12.5 embryonic cerebellum (frontal sections). The expression of markers of DNA synthesis (A,B: BrdU incorporation), cell division (C,D: phosphorylated histone H3), cell proliferation (E,F: Ki67) and cell-cycle control and exit (G,H: kip2) is not altered in the Ebf2 mutant cerebellar plate. The quantitation of BrdU-incorporating cells (I) and phosphorylated histone H3 positive cells (J) was obtained counting complete series of sections stained with the indicated antibodies. Statistical analysis of mean cell numbers and s.d. in +/+ versus null mutant embryos was conducted using a two-tailed t-test, equal variance. Scale bar: in H, 100 µm for A-H.