Fig. 4. Defective migration of a subset of PCs in the Ebf2 null cerebellum. (A-D) Immunohistochemistry with anti-CaBP antibody on sagittal paraffin-embedded sections, anterior to the left. At E15.5 many mutant PCs are abnormally retained in the anterior cortical transitory zone (ctz). (C,D) Higher magnification of insets in A,B, respectively. The results are representative of three experiments, each conducted in a different litter, comparing one mutant and one wt littermate. hb: hindbrain; mes: mesencephalon; vz: VZ; egl: external granular layer; chd: choroid plexus. Scale bar: in D, 200 µm for A,B; 50 µm for C,D.