Fig. 6. In the Ebf2 null cerebellum, the cell loss affects specific subtypes of PCs. All sections are frontal. E18.5 (A,B) and P4 (C-H) sections were immunoperoxidase-stained for neurogranin (A-F) and HSP25 (G,H) antibodies. In the mutant, at E18.5, neurogranin-positive PCs are located mostly near the VZ (arrow in B), and are selectively lost during development. At P4 neither neurogranin-positive (D,F) nor HSP25-positive PCs (H) are present in the mutant cortex. cf: climbing fibers. Scale bars: in F, 150 µm for A,B; 100 µm for C-F; in H, 200 µm for G,H.