Fig. 8. Ebf2-lacZ transgene expression in the heterozygote. (A) Wholemount X-gal staining of the adult cerebellum, posterior, dorsal and anterior views. I-IX: vermian lobules; l.ans, l.para: ansiform and paramedian lobules, respectively. In the vermis, transgene expression is absent from the NZ (e.g. lobule X) and CZ (e.g. lobule VIb), expressed in stripes in the CZ (e.g. lobule VIII) and in the AZ (e.g. lobule III; arrows: lacZ negative stripes). (B-H) transverse 40 µm sections double-labeled for the transgene (blue) and ZII (brown). (B) posterior hemisphere (crus II). The PC stripes P4a+ - P7+ are labeled [for stripe terminology, see Eisenman and Hawkes (Eisenman and Hawkes, 1993) and Sillitoe and Hawkes (Sillitoe and Hawkes, 2002)]. Transgene expression is restricted to P-stripes. Higher magnifications of the regions indicated are shown in D and E. (C) Posterior vermis. Lobule X and ventral lobule IX (the NZ) are comprised entirely of ZII+ PCs and there is no transgene expression. The NZ and PZ interdigitate in dorsal lobule IX and by lobule VIII a clear alternation of ZII+ (P1+ to P4+) and ZII-stripes is apparent. Ebf2-lacZ expression is restricted to P-stripes. A higher magnification view of the outlined area is illustrated in F. (G) Crus I shows transgene expression in the ZII- PC stripes. (H) Anterior vermis (AZ). ZII is expressed in an array of narrow stripes, P1+ at the midline, P2+ and P3+ laterally on either side. Transgene expression is in the ZII-immunonegative stripes. Scale bars: in A, 2 mm for A; in H, 1 mm for B,C,G,H; in F, 500 µm for D-F.