Fig. 8. The signal from the neural tube is not rhombomere 2-specific. (A) Scheme of neural tube electroporation at HH10. (B-G) Lateral views of electroporated embryos at HH19; anterior to the top, dorsal to the left. (B,E) The targeted area revealed by means of eGFP fluorescence. (C,D,F,G) Paraxis (C,F) or Myf5 (D,G) expression in blue and staining with the anti-GFP antibody in brown. Same embryo shown in B,C and E,F, respectively. (B-D) Control electroporation with the pCAß-IRES-eGFP vector lacking the open reading frame for Hoxb1 allows normal expression of Paraxis and Myf5. (E-G) Misexpression of Hoxb1 in the anterior hindbrain transforms the identity of rhombomeres into that of rhombomere 4 (r1^*, r2^*). However, Paraxis and Myf5 expression are not perturbed. Scale bar: 250 µm. d, diencephalon; hy, hyoid arch; m, midbrain; ma, mandibular arch; ov, otic vesicle; r, rhombomere; t, telencephalon.