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Figure 4


Fig. 4. pig-1 regulates the positioning of the cleavage plane in the HSB/PHB and Q.p neuroblasts. (A-E) pig-1 disrupts the size asymmetry of the daughters of the HSN/PHB neuroblast. The nuclear-localized reporter gmIs20 (hlh-14::gfp) was used to identify the daughters of the HSN/PHB neuroblast and measure the size of their nuclei (Frank et al., 2005). All images show a ventral view of a single embryo with anterior to the left. In each image, the anterior (arrow) and posterior (arrowhead) daughters of the HSN/PHB neuroblast are magnified in the inset. (A) Wild type, (B) pig-1(gm301), (C) ham-1(gm279) and (D) pig-1(gm301) ham-1(gm279). Scale bars: 5 µm. (E) Quantification of the ratio in nuclear diameter between the anterior and posterior daughters of the HSN/PHB neuroblast in multiple genotypes; n, number of pairs of neuroblast daughters scored. (F) Quantification of the ratio in cell size between the posterior and anterior daughters of the Q.p neuroblast; n, number of pairs of neuroblast daughters scored. The transcriptional reporter ayIs9 (Pegl-17::gfp) was used to identify and measure the size of the daughters of the Q.p neuroblast. Like the HSN/PHB neuroblast division, the Q.p neuroblast division is more symmetric in pig-1 mutants.